Anti-Phospho-JAK2 (Tyr1007/Tyr1008)

Code: 09-275 D2-231

Analysis Note

ControlLPS/IFN gamma untreated and treated RAW264.7 cell lysates

Application

Research CategorySignaling

Anti-phospho-JAK2 (Ty...


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Analysis Note

ControlLPS/IFN gamma untreated and treated RAW264.7 cell lysates

Application

Research CategorySignaling

Anti-phospho-JAK2 (Tyr1007/1008) Antibody is an antibody against phospho-JAK2 (Tyr1007/1008) for use in WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Janus kinase 2 (JAK2) is a ubiquitous non-receptor tyrosine kinase that interacts with multiple cell-surface receptors and non-receptor proteins to affect a range of cellular processes including immunity, cell growth, development, and differentiation. Among JAK2's receptor partners are the prolactin, growth hormone, thrombopoitin, and INF (α and β) receptors. JAK2 plays an important role in connecting the signaling pathways of these receptors to the activation of target genes. Upon ligand binding these receptors activate JAK2 inducing JAK2 autophosphorylation; This activated JAK2 in turn phosphorylates the cytoplasmic domains of these receptors providing a binding site for STAT transcription factors which are also phosphorylated by JAK2 leading to STAT protein dimerization and translocation to the nucleus to effect gene transcription. Aberrations in JAK2 expression and function may contribute to myelofibrosis and myelogenous leukemia.

Immunogen

KLH-conjugated linear peptide corresponding to human JAK2 phosphorylated at (Tyr1007/Tyr1008).

Epitope: Phosphorylated (Tyr1007/Tyr1008)

Linkage

Replaces: 04-1098

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, and 0.05% sodium azide.

Quality

Evaluated by Western Blot in LPS/IFN gamma untreated and treated RAW264.7 cell lysates.

Western Blot Analysis: A 1:5,000 dilution of this antibody detected JAK2 on 10 µg of LPS/IFN gamma untreated and treated RAW264.7 cell lysates.

Specificity

This antibody recognizes JAK2 phosphorylated at (Tyr1007/Tyr1008).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~130 kDa observed. Uncharacterized bands appear at ~48, 55, 65, and 100 kDa in some lysates.

antibody formaffinity isolated antibody
antibody product typeprimary antibodies
biological sourcerabbit
clonepolyclonal
Gene Informationchicken ... Jak2(374199)frog ... Jak2(100036599)human ... JAK2(3717)mouse ... Jak2(16452)pig ... Jak2(397201)rat ... Jak2(24514)
NCBI accession no.NP_004963
purified byaffinity chromatography
Quality Level100
shipped inwet ice
species reactivity (predicted by homology)rat (based on 100% sequence homology), zebrafish (based on 100% sequence homology), Xenopus (based on 100% sequence homology), porcine (based on 100% sequence homology), chicken (based on 100% sequence homology), human (based on 100% sequence homology)
species reactivitymouse, pig
technique(s)western blot: suitable
UniProt accession no.O60674
This product has met the following criteria to qualify for the following awards:



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